CD4<sup>+</sup>CD25<sup>+</sup>调节性T细胞检测在急性髓细胞白血病中的临床意义

陶贞霞; 郭晓玲; 孟宇; 于彦居; 丁继生; 王娟

CD4⁺CD25⁺调节性T细胞检测在急性髓细胞白血病中的临床意义

- 1. 沧州市中心医院中心实验室(河北沧州, 061001);
- 2. 河北医科大学第二医院河北省血液病研究所
基金项目:
河北省自然科学基金（No：C2008001097）；国家高等学校博士学科点专项科研基金（No：200800890011）

详细信息

通讯作者: 郭晓玲,E-mail:13932113351@163.com

中图分类号: R733.71

收稿日期: 2012-12-26

Clinical significance of CD4⁺CD25⁺ regulatory T cells detection in acute myelocytic leukemia

- 1. Central Laboratory, Cangzhou Central Hospital, Cangzhou, 061001, China;
- 2. the Second Hospital of Hebei Medical University, Blood Institute in Hebei Province

More Information

Corresponding author: GUO Xiaoling, E-mail: 13932113351@163.com

Received Date: 26 December 2012

摘要

摘要: 目的：探讨CD4⁺CD25⁺调节性T细胞检测在急性髓细胞白血病（AML）中的临床意义。方法：选取AML患者41例和体检健康者16例，用流式细胞仪检测CD4⁺CD25⁺调节性T细胞占CD4⁺T细胞的比例。结果：AML患者外周血的CD4⁺CD25⁺调节性T细胞表达明显高于对照者[（3.88±1.99）%：（1.96±1.66）%，P<0.01]。AML组内，化疗耐药者（15例）的CD4⁺CD25⁺调节性T细胞表达显著高于化疗敏感者（14例）[（3.90±1.89）%：（2.00±1.65）%，P<0.01]。14例化疗敏感者中，缓解后复发者（4例）的CD4⁺CD25⁺调节性T细胞表达显著高于持续缓解者（10例）[（3.95±1.86）%：（2.02±1.55）%，P<0.05]。结论：CD4⁺CD25⁺调节性T细胞可能诱导免疫耐受，从而导致AML细胞的恶性增殖，影响白血病化疗的疗效，也可能是引起缓解患者复发的原因之一。
- 急性髓细胞白血病 /
- CD4⁺CD25⁺调节性T细胞 /
- 预后 /
- 免疫表型 /
- 流式细胞术
Abstract: Objective: To explore the clinical significance of CD4⁺CD25⁺ regulatory T cells detection in acute myelocytic leukemia (AML).Method: The percentage of CD4⁺CD25⁺ regulatory T cells in CD4⁺ T cells was detected in 41 AML and 16 normal controls by flow cytometry.Result: CD4⁺CD25⁺ regulatory T cells expression in AML patients was significantly higher than that in normal controls (P<0.01).CD4⁺CD25⁺ regulatory T cells expression in 15 patients with chemotherapy tolerance was significantly higher than that in 14 patients with chemotherapy sensitivity (P<0.01).CD4⁺CD25⁺ regulatory T cells expression in 4 patients with recurrence was significantly higher than that in 10 patients with sustained remission (P<0.05).Conclusion: CD4⁺CD25⁺ regulatory T cells may induce immune tolerance and lead to acute leukemia cell proliferation and influence the curative effect of leukemia chemotherapy.CD4⁺CD25⁺ regulatory T cells may be one of the reasons which resulted in the recurrence of patients after remission.
- acute myelocytic leukemia /
- CD4⁺CD25⁺ regulatory T cells /
- prognosis /
- immunophenotype /
- flow cytometry

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参考文献(10)

[1]	徐文鑫,翁开枝.免疫细胞在白血病免疫治疗应用中的研究进展[J].医学综述,2006,12(12):739-742.
[2]	张之南.血液病诊断及疗效标准[M].3版.北京:科学出版社,2007:131-133.
[3]	WANG X,ZHENG J,LIU J,et al.Increased population of CD4(+)CD25(high),regulatory T cells with their higher apoptotic and proliferating status in peripheral blood of acute myeloid leukemia patients[J].Eur J Haematol,2005,75:468-476.
[4]	BEYER M,KOCHANEK M,DARABI K,et al.Reduced frequencies and suppressive function of CD4⁺CD25 high regulatory T cells in patients with chronic lymphocytic leukemia after therapy with fludarabine[J].Blood,2005,106:2018-2025.
[5]	张帆,徐菁,朱镭.血液系统恶性肿瘤患者CD4⁺CD25⁺调节性T细胞的变化及意义[J].山西医科大学学报,2007,38(12):1106-1108.
[6]	RALAINIRINA N,POLI A,MICHEL T,et al.Control of NK cell functions by CD4⁺CD25⁺ regulatory T cells[J].J Leukoc Biol,2007,81:144-153.
[7]	陆春伟,佟海侠,陆美言.急性白血病患者外周血淋巴细胞亚群和调节性T细胞的检测及临床意义[J].现代肿瘤医学,2010,18(11):2230-2233.
[8]	杨云,何爱丽,张王刚,等.急性白血病患者调节性T细胞的检测及临床意义[J].临床血液学杂志,2010,23(3):71-73.
[9]	SZCZEPANSKI M J,SZAINIK M,CZYSTOWSKA M,et al.Increased frequency and suppression by regulatory T cells in patients with acute myelogenous leukemia[J].Clin Cancer Res,2009,15:3325-3332.
[10]	张爱梅,翟志敏,王宁玲,等.CD4⁺CD25高表达调节性T细胞与儿童急性淋巴细胞白血病的关系[J].白血病.淋巴瘤,2008,17(4):267-270.