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摘要: 目的：探讨溶血标本对电化学发光法检测血清抗环瓜氨酸肽抗体（Anti-CCP）的干扰作用。方法：依据美国临床实验室标准化委员会制定EP7-P文件标准程序，取10例正常人新鲜血液分离血清及其红细胞，配对后配制成不同浓度的溶血模型，检测血红蛋白浓度与溶血指数之间的关系。分析溶血指数与电化学发光法检测血清Anti-CCP的关系，并且检测56例类风湿关节炎（RA）患者、35例其他风湿性疾病患者及30名健康对照者血清中的Anti-CCP，观察标本血清Anti-CCP检测结果的分布。结果：血红蛋白浓度与溶血指数在临床标本溶血范围内呈良好的线性关系，Y（溶血指数）=108.4X（血红蛋白浓度）-4.1，R2=0.990 6。24例正常人标本的不同溶血程度对Anti-CCP检测结果产生正干扰。RA患者血清Anti-CCP水平显著高于健康对照者，并且Anti-CCP临界值少见。结论：溶血干扰电化学发光法检测血清Anti-CCP结果，且Anti-CCP是观察判断RA患者一项较好的参考指标。Abstract: Objective: To evaluate the interference effect of hemolysis on Anti-cyclic cirullinated peptide antibodies (Anti-CCP) tested by electrochemiluminescence.Method: According to the document EP7-P set by Clinical and Laboratory Standards Institute (CLSI),10 fresh blood samples from health adults were collected and the isolated erythrocytes and serum were prepared to hemolytic models with multistep levels of concentration.The relationship between the concentration of Hb and hemolytic index (HI) was analyzed.The interference effect of IH on Anti-CCP by means of Electrochemiluminescence was evaluated.The serum level of Anti-CCP in 56 patients with rheumatoid arthritis (RA),35 other rheumatic disease and 30 health adults were compared,and the distribution of Anti-CCP level was analyzed.Result: There was a good linear correlation between Hb concentration and IH in the clinical specimen hemolysis range,Y(IH)=108.4X(Hb concentration)-4.1,R2=0.990 6.Different hemolysis levels of specimens from 24 health adults had a positive interference on Anti-CCP results.The serum Anti-CCP level of RA patients was significantly higher than that of health adults.Conclusion: Hemolysis would interfere the detection of Anti-CCP by Electrochemiluminescence and Anti-CCP would be a reference index for RA patients.
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Key words:
- electrochemiluminescence /
- Anti-CCP /
- hemolysis /
- rheumatoid arthritis
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