PCR-SSP法检测吉林省汉族人群血小板抗原1~6系统研究

张冬霞; 黄氏敏; 于红; 李颖; 史立英; 刘冰

PCR-SSP法检测吉林省汉族人群血小板抗原1~6系统研究

- 1. 吉林省肿瘤医院输血科(长春, 130012);
- 2. 吉林大学第一医院输血科
基金项目:
吉林省卫生厅课题（No：2009z029）

详细信息

通讯作者: 刘冰,E-mail:liubing1204@163.com

中图分类号: R596

收稿日期: 2012-07-12

Study on genotyping of HPA1~6 system by PCR-SSP in Jilin Han population

- 1. Department of Blood Ttransfusion, Jilin Province Cancer Hospital, Changchun, 130012, China;
- 2. Department of Blood Transfusion, the First Hospital, Jilin University

More Information

Corresponding author: LIU Bing, E-mail: liubing1204@163.com

Received Date: 12 July 2012

摘要

摘要: 目的：应用PCR-SSP法对吉林省汉族人群人血小板同种抗原系统（HPA）1~6进行基因及其多态性分布特征研究。方法：用DNA试剂盒提取外周血标本中DNA，通过特异性引物（PCR-SSP）扩增HPA等位基因。检测200名HPA1~6抗原系统共12个抗原的基因分型。结果：200名无偿献血并无血缘关系的吉林省汉族人群HPA基因频率为：HPA-1a 0.990 0、1b 0.010 0；2a 0.930 0、2b 0.070 0；3a 0.557 5、3b 0.442 5；4a 1.000 0、4b 0.000 0；5a 0.990 0、5b 0.010 0；6a 0.987 5、6b 0.012 5。结论：吉林地区血小板志愿捐献者HPA1~6抗原系统的基因频率符合Hardy-Weinberg遗传定律，与其他地区和国家的同类资料相比显示出种族和地域性差异，由此建立起了吉林地区已知HPA基因型的血小板捐献者资料库。
- PCR-SSP法 /
- 人血小板同种抗原 /
- 基因频率
Abstract: Objective: To analyze the gene polymorphism distribution characteristics of human platelet antigen(HPA)1~6 using polymerase chain reaction with sequence specific primers (PCR-SSP) in Jilin Han population.Method: DNA was extracted from peripheral blood samples from 200 Han donors in Jilin Province by DNA kit and PCR-SSP was introduced to analyze the genotypes of HPA 1~6.Result: HPA allele frequencies of 200 Han donors in Jilin Province were HPA-1a 0.9900,1b 0.0100;2a 0.9300,2b 0.0700;3a 0.5575,3b 0.4425;4a 1.0000,4b 0.0000;5a 0.9900,5b 0.0100;6a 0.9875,6b 0.0125.Conclusion: HPA allele frequencies of Han population in jilin province fit Hardy-Weinberg equilibrium,and compared with other regions and countries,they had race and regional differences.Thus a database of platelet donors with known HPA genotype was established in Jilin province.
- PCR-SSP /
- human platelet antigen /
- gene frequency Jilin province

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